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Title:Cryo EM reconstruction of mammalian 80S ribosome in classic PRE state 2
Authors:Budkevich T, Giesebrecht J, Altman R, Munro J, Mielke T, Nierhaus K, Blanchard S, Spahn C
Sample:80S-PRE-classic-2 complex from rabbit (Oryctolagus cuniculus) liver
Method:Single particle reconstruction (9 angstroms resolution)
Red flagLatest update:2013-03-06
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Sample name: 80S-PRE-classic-2 complex from rabbit (Oryctolagus cuniculus) liver
Oligomeric state: monomer
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1ribosome-eukaryote80S ribosomefalseOryctolagus cuniculus
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mLQuantifoil grids
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%77 KFEI VITROBOTFlash-frozen in liquid ethane on carbon coated Quantifoil grids msVitrification instrument: VitroBot (FEI)
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI POLARA 300300 kVSPOT SCANBRIGHT FIELD2 mm2000 nm4000 nm3900065520FIELD EMISSION GUNKODAK SO-163 FILM mmObjective lens astigmatism was corrected at 200,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
CartridgeGATAN HELIUM°° eV77 K K K mrad20 e/Å217-OCT-2006
Protocol:Multi-particle refinement
CTF correction:By defocus groups
Number of particles:81946
Imposed symmetry:C1
Resolution by author:9 Å
Resolution method:FSC 0.5
Processing details:Several rounds of multireference 3D projection refinement.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
874.7 μm/pixelPRIMESCAN
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails