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Title:Structure of late pre-60S ribosomal subunits with nuclear export factor Arx1 bound at the peptide exit tunnel
Authors:Bradatsch B, Leidig C, Granneman S, Gnaedig M, Tollervey D, Boettcher B, Beckmann R, Hurt E
Sample:pre-60S particle
Method:Single particle reconstruction (11.9 angstroms resolution)
Red flagLatest update:2012-11-28
Sample
Sample name: pre-60S particle
Theoretical molecular weight of the sample: 1.3
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1ribosome-eukaryotePre-60S particlefalseSaccharomyces cerevisiae
2proteinProliferation-associated protein 2G4falseHomo sapiens
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7 mg/mL50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1.5 mM or 5 mM MgCl2, 0.075% (v/v) NP-40
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE95% KFEI VITROBOT MARK IVBlot for 2 seconds before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS200 kVFLOOD BEAMBRIGHT FIELD mm nm nm95000FIELD EMISSION GUNGENERIC CCD mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
FEI TITAN KRIOS AUTOGRID HOLDER°° eV K K K mrad20 e/Å206-APR-2012
Processing
Protocol:Projection Matching
Software:Signature, Spider, Chimera
CTF correction:Subvolumes
Number of particles:100000
Imposed symmetry:C1
Resolution by author:11.9 Å
Resolution method:FSC 0.5
Processing details:The particles were selected using an automatic selection program.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails