top_cornerHomeEMD-5520  Contact EMDataBank 
Image unavailable
Title:CryoEM structure of Dengue virus, full map
Authors:Zhang X, Ge P, Yu X, Brannan JM, Bi G, Zhang Q, Schein S, Zhou ZH
Sample:Dengue Virus 2
Method:Single particle reconstruction (3.6 angstroms resolution)
Red flagLatest update:2013-01-16
Other Views:
Sample
Sample name: Dengue Virus 2
Oligomeric state: full viral capsid
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusDengue virus 2Dengue virus 2
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.4 mg/mL50 mM Tris, 140 mM NaCl, 5 mM EDTAQuantifoil R2/1
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER2.5 uL sample added per grid ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS300 kVFLOOD BEAMBRIGHT FIELD2.7 mm500 nm2400 nm5900057518FIELD EMISSION GUNKODAK SO-163 FILM mmsoftware compensation

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
FEI TITAN KRIOS AUTOGRID HOLDER°° eV K K K mrad25 e/Å223-DEC-2010
Processing
Protocol:MPSA
Software:EMAN
CTF correction:EMAN per particle, with astigmatism compensation
Number of particles:9288
Imposed symmetry:I
Resolution by author:3.6 Å
Resolution method:FSC 0.143
Processing details:EMAN with Multi-path Simulated Annealing.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
11036.35 μm/pixelNIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails