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Title:Yeast 20S proteasome in complex with purified yeast 19S base sub complex
Authors:Li X, Kim HM, Cheng Y
Sample:Reconstituted complex of Yeast 20S + 19S base sub complex
Method:Single particle reconstruction (9.6 angstroms resolution)
Red flagLatest update:2013-06-05
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Sample
Sample name: Reconstituted complex of Yeast 20S + 19S base sub complex
Oligomeric state: one 19S base subcomplex binds to a 20S core particle
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinproteasomefalseSaccharomyces cerevisiaereconstituted complex of yeast 20S core particle with purified 19S base sub complex
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%120 KFEI VITROBOT MARK III ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm1200 nm2100 nm100000FIELD EMISSION GUNTVIPS TEMCAM-F816 (8k x 8k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
CT3500GATAN LIQUID NITROGEN eV K K K mrad25 e/Å2Low-dose procedure01-JAN-2010
Processing
Software:FREALIGN
CTF correction:Each Particle
Number of particles:51000
Imposed symmetry:C1
Resolution by author:9.6 Å
Resolution method:FSC 0.143
Processing details:FREALIGN.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1RYP rigid bodyChimeraREAL