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Title:Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, dissociated S1-ACE2
Authors:Gui M, Song W
Sample:Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, dissociated S1-ACE2
Method:Single particle reconstruction (11.6 angstroms resolution)
Red flagLatest update:2018-08-22
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Sample
Sample name: Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, dissociated S1-ACE2
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinTrypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, dissociated S1-ACE2false
2proteinS1 subunit of trypsin-cleaved and low pH-treated SARS-CoV spikefalseSARS coronavirus
3proteinhuman ACE2falseHomo sapiens
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
5.8 mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS300 kVFLOOD BEAMBRIGHT FIELD mm nm nmFIELD EMISSION GUNGATAN K2 SUMMIT (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
OTHER°° eV K K K mrad50.0 e/Å2
Processing
Number of particles:41289
Imposed symmetry:C1
Resolution by author:11.6 Å
Resolution method:FSC 0.143 CUT-OFF
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails